NASA 1996 SBIR Phase I


PROPOSAL NUMBER : 96-1 17.03-9591

PROJECT TITLE : Multistage Electrophoretic Purification of Cells, Particles, and Proteins

TECHNICAL ABSTRACT (LIMIT 200 WORDS)

Medical research in space provides the impetus for advancing a novel process for free-fluid electrophoretic purification of living cells and proteins. Exploiting flight-proven multistage technology developed by SHOT, the process separates single cells in useful quantities and at high concentrations. The isothermal process depends on the electrophoretic mobility of separands, and is gravitationally stabilized so that it functions in laboratories both on earth and in space. The proposed innovation is a multistage, thin-layer, free-electrophoresis separator for purifying cells, particles, and proteins. The instrument employs palladium electrodes to create uniform electric fields for the high-resolution separation of organic materials. This research effort is expected to advance the technology needed to fully understand the advantages of the process and to develop the innovation into commercial products. Our Phase I objectives include developing: a mass transfer model, innovative methods for dealing with electrolysis, techniques for transporting and varying electrical energy, and approaches for keeping the process isothermal. Phase I research is expected to establish the technical and ommercial feasibility of the innovation. Further development during Phases II and III is anticipated to yield commercially marketable gravity-independent separators, suitable for medical research applications in analytical laboratories on earth and on the International Space Station.
POTENTIAL COMMERCIAL APPLICATIONS
A bench-scale laboratory separator will be one of the first products commercialized for medical and biotechnology applications. Such an instrument will find specific applications in immunological research and for protein purification. Other products and services are expected to have broad application in numerous scientific research and medical areas; hence, the market for our technology is the widely segmented biomedical field. While our technology will have broad global applications in the ground- based markets, our target market niche is application of the technology in the unique environment of space. Our commercial products will take advantage of the widespread need for unique separation techniques and the low gravity environment of space.
NAME AND ADDRESS OF PRINCIPAL INVESTIGATOR
John T. Weber
SHOT, Inc.
5605 Featherengill Road
Floyds Knobs, IN 47119
NAME AND ADDRESS OF OFFEROR
SHOT, Inc.
5605 Featherengill Road
Floyds Knobs, IN 47119