Project Title:

PIN - A programmable incubator for autonomous support of living cells in microgravity

Synergetics, Inc.
1831 Lefthand Circle
Longmont, CO 80501

93-1  15.05 5200    __      AMOUNT REQUESTED $70,000

PIN - A programmable incubator for autonomous support of living cells in microgravity

Abstract:

The instrument described in this proposal, the programmable
incubator (PIN), is a low-cost, highly reusable and reprogrammable
cellular life support module for the study of single celled
organisms such as bacteria, algae, and yeast, and for the study of
plant and animal cell cultures in a microgravity environment. This
device maintains cells under controlled environmental conditions
autonomously for periods of up to 90 days, and allows pre-
programmed and event-driven actions to be executed such as the
addition of fixatives, replenishment of nutrients, or the
initiation of new cultures. The PIN provides measurement of
temperature and loads, pH, optical density, video microscopy, and
spectral analysis of cultures. Fluid transfers between cell
cultures and other support cultures is by means of disposable
needles. One unique aspect of the PIN is the ability to totally
simulate its activities on a standard PC or Macintosh without
access to the actual hardware, allowing end-users to program the
PIN and test experimental regimes. The closure and multiple levels
of containment reduce safety concerns during spaceflight and open
terrestrial uses requiring containment where hazardous agents are
to be studied. Cleanup and refurbishment are easily achieved with
no risk to the PIN operator.

The PIN adds to the commercial potential of space-based biological
research by supplying a reliable yet low-cost access vehicle for
biotechnology companies. The PIN may also be used for ground-based
pre-flight simulations, and for the autonomous handling of
dangerous or infectious materials in the laboratory.

MICROGRAVITY CELLS INCUBATOR PROGRAMMABLE AUTONOMOUS