NASA SBIR 2017 Solicitation


PROPOSAL NUMBER: 171 H3.02-9356
SUBTOPIC TITLE: Environmental Monitoring for Spacecraft Cabins
PROPOSAL TITLE: A 3D Printer Enabled, High Performing, Microgravity Compatible, and Versatile Sample Preparation Platform

SMALL BUSINESS CONCERN (Firm Name, Mail Address, City/State/Zip, Phone)
AI Biosciences, Inc.
1902 Pinon Dr, STE C
COLLEGE STATION, TX 77845 - 5816
(979) 268-1091

PRINCIPAL INVESTIGATOR/PROJECT MANAGER (Name, E-mail, Mail Address, City/State/Zip, Phone)
Dr. Season Wong
1902 Pinon Dr, STE C
COLLEGE STATION, TX 77845 - 5816
(979) 268-1091

CORPORATE/BUSINESS OFFICIAL (Name, E-mail, Mail Address, City/State/Zip, Phone)
Dr. Season Wong
1902 Pinon Dr, STE C
COLLEGE STATION, TX 77845 - 5816
(979) 268-1091

Estimated Technology Readiness Level (TRL) at beginning and end of contract:
Begin: 4
End: 6

Technology Available (TAV) Subtopics
Environmental Monitoring for Spacecraft Cabins is a Technology Available (TAV) subtopic that includes NASA Intellectual Property (IP). Do you plan to use the NASA IP under the award?

TECHNICAL ABSTRACT (Limit 2000 characters, approximately 200 words)
AI Biosciences proposes to demonstrate the ability of a microgravity-compatible, compact, light-weight, and automated versatile sample preparation platform (VSPP) that processes samples from various sample matrices (swab, potable water, blood, urine, etc.) to yield high quality nucleic acids for downstream molecular detection and identification in a closed-cartridge system. Its primary function will enable NASA to rapidly identify microorganisms that could affect crew safety. This near-term deployable cartridge and platform system also has the option to perform isothermal and PCR amplification of nucleic acids. This highly flexible system will allow previously complicated, labor-intensive, and time consuming processes to be carried out by a turn-key and closed system using pre-filled cartridges. We have demonstrated on the ground in a laboratory setting that the entire setup for high quality DNA/RNA extraction from urine, whole blood, serum, and saliva can be achieved via simple and reversible retrofitting of a low-cost fused deposition modeling (FDM) based 3D printers ranging from $199 to $750. The VSPP can also be used to capture and purify cell and protein targets. The ability of having a dual-use 3D printer in-flight to carry out sample preparation and additive manufacturing in-flight can be an attractive option in long duration space flights.

POTENTIAL NASA COMMERCIAL APPLICATIONS (Limit 1500 characters, approximately 150 words)
The ability to carry out sample preparation in microgravity using a small footprint, versatile device and extensible cartridges in an enclosed format will introduce the ability to carry out sample preparation for molecular biology tests from a variety of sample matrices (cell culture, plant tissue, blood, swab, etc.). The unit can also be easily coupled with suitable downstream detection modules to expand its capabilities to include processes such as PCR, isothermal amplification, and sequencing. With the development of proper assays, new applications can be extended to include microbial detection in advanced life support systems, in-vitro testing for infectious diseases during space flight, gene expression analysis for radiation exposure monitoring/studies, and effect of space flight on microbial gene expression and virulence. These processes can be completed inside the Microgravity Sciences Glovebox (MSG) or disposable glove bag to minimize sample transfer or contamination concerns. By offering a medium throughput sample preparation platform, our VSPP unit will enable researchers and companies to carry out in-situ life sciences research inside the ISS with near real-time result capability when coupled with appropriate detection systems.

POTENTIAL NON-NASA COMMERCIAL APPLICATIONS (Limit 1500 characters, approximately 150 words)
With advances in molecular diagnostics, life science, and forensics, there will be a vast increase in demand for instrumentation that can handle automated sample preparation in a closed format to eliminate cross-contamination. According to a recent report by Markets and Markets, the global nucleic acid isolation and purification market is expected to reach $3.8 billion USD by 2020. Increasing healthcare expenditure will aid the growth of the nucleic acid isolation and purification market. Our unit also has the capability to carry out molecular detection by isothermal amplification. We see it as a great opportunity to offer at-home healthcare diagnostics. Other applications of this VSPP include environmental monitoring, agriculture, and food production safety.

TECHNOLOGY TAXONOMY MAPPING (NASA's technology taxonomy has been developed by the SBIR-STTR program to disseminate awareness of proposed and awarded R/R&D in the agency. It is a listing of over 100 technologies, sorted into broad categories, of interest to NASA.)
Biological (see also Biological Health/Life Support)
Chemical/Environmental (see also Biological Health/Life Support)
Health Monitoring & Sensing (see also Sensors)

Form Generated on 04-19-17 12:59