NASA SBIR 2016 Solicitation

FORM B - PROPOSAL SUMMARY


PROPOSAL NUMBER: 16-1 H3.01-7134
SUBTOPIC TITLE: Environmental Monitoring
PROPOSAL TITLE: Devices and Methods for Collection and Concentration of Air and Surface Samples for Improved Detecti

SMALL BUSINESS CONCERN (Firm Name, Mail Address, City/State/Zip, Phone)
InnovaPrep, LLC
132 East Main Street
Drexel, MO 64742 - 0068
(816) 619-3375

PRINCIPAL INVESTIGATOR/PROJECT MANAGER (Name, E-mail, Mail Address, City/State/Zip, Phone)
Mr Andrew E Page
apage@innovaprep.com
132 East main street
Drexel, MO 64742 - 0068
(816) 619-3375 Extension :129

CORPORATE/BUSINESS OFFICIAL (Name, E-mail, Mail Address, City/State/Zip, Phone)
Ms Ann K Packingham
apack@innovaprep.com
132 East Main Street
Drexel, MO 64742 - 0068
(816) 619-3375 Extension :109

Estimated Technology Readiness Level (TRL) at beginning and end of contract:
Begin: 3
End: 4

Technology Available (TAV) Subtopics
Environmental Monitoring is a Technology Available (TAV) subtopic that includes NASA Intellectual Property (IP). Do you plan to use the NASA IP under the award?
No

TECHNICAL ABSTRACT (Limit 2000 characters, approximately 200 words)
Protecting the International Space Station (ISS) crew from microbial contaminants is of great importance. Bacterial and fungal contamination of air, surfaces and water aboard ISS has the potential to cause sickness among ISS crew and to impact onboard experiments. Further, it has been noted that pathogenicity and virulence of microbes can increase in microgravity environments. These factors, along with the high consequence of sickness in the remote space environment, creates a significant need for a rapid way to determine when microbial contamination events occur. To this end, InnovaPrep LLC of Drexel, MO proposes development of improved methods for collection of microbes from air and surfaces for delivery into a small liquid volume compatible with advanced molecular based detection systems.
Rapid microbiological detection systems have taken dramatic steps forward in the last two decades and today detection of even a single organism is possible in less than one hour. Unfortunately, development of rapid detection methods has far outpaced development of sample collection and concentration techniques, which are necessary to enable detection of low microbial concentrations in the environment. In the proposed Phase I work, InnovaPrep will leverage current aerosol and surface collection and elution technologies and innovations from a 2015 NASA awarded SBIR for microbial concentration from ISS potable water, for handling of these technologies in a microgravity environment, to develop novel ISS aerosol and surface collection systems. Specifically, InnovaPrep will develop new, optimized aerosol filter assemblies and surface sampling assemblies that allow for collection from larger air volumes and surface areas and elution into smaller liquid volumes than is currently possible.

POTENTIAL NASA COMMERCIAL APPLICATIONS (Limit 1500 characters, approximately 150 words)
Protecting the ISS crew from microbial contaminants is of great importance. Bacterial and fungal contamination of air, surfaces and water aboard ISS has the potential to cause sickness among ISS crew and to impact onboard experiments. Further, as noted by Yamaguchi et al., crewed habitats in space can significantly alter the relationships between humans and microbes, including increasing the pathogenicity and virulence of microbes. The possible high penalty of sickness while aboard the ISS creates a significant need for a rapid way to determine when microbial contamination events occur. To this end, InnovaPrep proposes development of improved methods for collection of microbes from air and surfaces for delivery into a small liquid volume compatible with advanced molecular based detection systems. In 2015, InnovaPrep was awarded a Phase I SBIR for development of a microbial concentration system for ISS potable water and, after what by most measures was a successful Phase I project, is currently awaiting word on a potential Phase II award. This system is based on membrane filtration of water samples and Wet Foam Elution of the captured particles. This method was developed by InnovaPrep as a means for efficiently eluting microbial particles from large membrane filters and quickly led InnovaPrep to apply this technique to elution of microbes from aerosol collection filters and swabs, wipes and other surface sampling instruments with significant success.

POTENTIAL NON-NASA COMMERCIAL APPLICATIONS (Limit 1500 characters, approximately 150 words)
InnovaPrep has applied this wet foam elution technique to elution of microbes from aerosol collection filters and swabs, wipes and other surface sampling instruments with significant success. Specifically, with regard to aerosol collection, the company has developed a family of aerosol collectors that utilize electret filter collection and wet foam elution, called the Bobcat, has quickly become the go to collector for ongoing Department of Defense (DoD) biodefense detection programs. An integratable version of the Bobcat is the selected collector for the DoD Joint Biological Tactical Detection System program and is also being used in several configurations by three of the large scale integrators on the ongoing DoD Joint United States Forces Korea Portal and Integrated Threat Recognition (JUPITR) program. Further, the underlying technology was also recently selected for award under the Department of Homeland Security SenseNet program as part of a team selected to develop an autonomously operated bioterrorism detection system. InnovaPrep is also in discussions with multiple commercial partners about the potential of developing a very low cost collector based on these same technologies that would be used for microbial monitoring in the agricultural and pharmaceutical industries.In the area of surface collection, InnovaPrep has more recently engaged in development of a range of surface collection devices for Animal Health, DoD and Food Safety.

TECHNOLOGY TAXONOMY MAPPING (NASA's technology taxonomy has been developed by the SBIR-STTR program to disseminate awareness of proposed and awarded R/R&D in the agency. It is a listing of over 100 technologies, sorted into broad categories, of interest to NASA.)
Biological (see also Biological Health/Life Support)
Health Monitoring & Sensing (see also Sensors)

Form Generated on 04-26-16 15:14