NASA STTR 2007 Solicitation

FORM B - PROPOSAL SUMMARY


PROPOSAL NUMBER: 07-2 T3.02-9983
PHASE 1 CONTRACT NUMBER: NNX08CD36P
RESEARCH SUBTOPIC TITLE: Bio-Technology and Life Support
PROPOSAL TITLE: An On-Chip Nano-Plasmonics Based Urine Protein Assay Cartridge

SMALL BUSINESS CONCERN (SBC): RESEARCH INSTITUTION (RI):
NAME: CFD Research Corporation NAME: University of Pittsburgh
STREET: 215 Wynn Drive, 5th Floor STREET: 219 Parkman Avenue
CITY: Huntsville CITY: Pittsburgh
STATE/ZIP: AL  35805 - 1926 STATE/ZIP: PA  15260 - 3900
PHONE: (256) 726-4800 PHONE: (412) 624-8430

PRINCIPAL INVESTIGATOR/PROJECT MANAGER (Name, E-mail, Mail Address, City/State/Zip, Phone)
Silvia Harvey
sxh@cfdrc.com
215 Wynn Dr.
Huntsville, AL 35805 - 1944
(256) 256-4858

Expected Technology Readiness Level (TRL) upon completion of contract: 4 to 5

TECHNICAL ABSTRACT (Limit 2000 characters, approximately 200 words)
Long-term exposure to microgravity and radiation during space exploration can pose a critical threat to the health of a flight crew. Real-time monitoring of urine protein levels is an effective way to follow the onset and progress of many diseases and guide the prompt selection of proper therapy. The success of such diagnostic tasks, which are vital for future space missions, critically depends upon the degree of automation, reusability and long-term lifetime of such trace level detection devices. To meet this need, this program will develop a novel on-chip, reagent-free, nano-plasmonic sensor cartridge to concurrently quantify the presence of different urine proteins. The envisioned device is compact, lightweight, fully integrated and automated, and highly cost- and power-effective. In Phase I, a new nano-plasmonics chip technology-based SPR sensor has been developed and successfully demonstrated for protein detection with excellent sensitivity. Critical microfluidic components, used in sample preparation, and adaptable to the sensor chip have been developed and validated by multi-physics-based simulations and modeling. In Phase II, the nano-plasmonic sensor will be optimized to increase sensitivity, stability, and the response to regular urine samples. The sensor chip will be integrated with the system-engineered microfluidics network to form a seamless urine protein assay cartridge (PAC) that enables automated surface modification, sample pretreatment, analyte detection and sensor regeneration for reuse. We will use modular design of the PAC that is compatible with existing NASA astrobiological instruments and will enable high-throughput proteome profiling analysis.

POTENTIAL NASA COMMERCIAL APPLICATIONS (Limit 1500 characters, approximately 150 words)
NASA will have a reusable on-chip nano-plasmonics based urine protein assay cartridge (PAC) that can be easily integrated with existing astrobiological instrumentation to keep track of astronaut health during planetary exploration. In addition, the same device can be adapted and used in other applications such as, life discovery on other planets, pharmacotherapy environment monitoring, and space biology experiments.

POTENTIAL NON-NASA COMMERCIAL APPLICATIONS (Limit 1500 characters, approximately 150 words)
The platform developed in this effort will provide the technological backbone to develop microfluidic processing systems and nano-biosensors for a variety of applications in healthcare and the life sciences. This platform will enable the creation of analytical tools for the preparation, detection, and analysis of low abundance proteins obtained from physiological fluids and cells. It may find use in drug discovery and the study of human diseases, clinical and preclinical diagnosis, as well as in the areas of proteomics, genomics, and homeland security. Total market estimates exceed several hundred million dollars.

NASA's technology taxonomy has been developed by the SBIR-STTR program to disseminate awareness of proposed and awarded R/R&D in the agency. It is a listing of over 100 technologies, sorted into broad categories, of interest to NASA.

TECHNOLOGY TAXONOMY MAPPING
Biochemical
Biomedical and Life Support
Biomolecular Sensors
Optical
Portable Data Acquisition or Analysis Tools


Form Generated on 02-10-09 12:09